Molecular simulations often require tailored groups of atoms for detailed analysis or specialized workflows, such as pulling or custom restraints. Did you know that GROMACS Wizard in SAMSON allows you to create custom index groups? This feature empowers you to go beyond the default groups automatically generated by GROMACS, providing more flexibility for complex simulations. Let’s explore how you can leverage this feature to enhance your workflow.
Why Create Custom Index Groups?
By default, GROMACS generates index groups based on molecular components, such as proteins, solvent, or ions. While useful, these pre-defined groups may not align with the goals of every simulation. Custom index groups allow you to define tailored atom selections, aiding in analysis and simulation tasks like pulling simulations, computing specialized interactions, or defining specific regions of interest.
How to Add Custom Index Groups in GROMACS Wizard
The process of creating custom index groups in GROMACS Wizard is straightforward and integrates seamlessly with SAMSON’s visualization and selection tools. Here’s a step-by-step guide:
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Click the Add/edit index groups button in the GROMACS Wizard interface.

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A pop-up window will appear where you can define your new index groups.

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Select atoms in the SAMSON interface using its comprehensive selection mechanisms. For example, you can select residues part of alpha helices via Select > Residues > Amino acids > Secondary structure > Alpha helices.

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In the Index Groups window, click the Generate based on current selection in document button. SAMSON will automatically generate a GROMACS-compatible selection string for the selected atoms.

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Test and refine your selection if needed, then assign a name to the new group (e.g.,
HELICES). -
Click the Add index group to the list button to save your custom group.

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Your new index group will appear in the list, ready to be used in subsequent simulation steps or analyses.

Practical Example: Index Groups for Alpha Helices
Let’s consider a practical example: creating an index group for residues forming alpha helices. This group could later serve as a pull coordinate group or be analyzed to understand changes in secondary structure during simulation. By using SAMSON’s visualization features and GROMACS Wizard’s ability to auto-generate selection strings, you reduce the room for error and save significant time compared to manual setups.
Key Benefits
- Precision: Create highly specific atom selections tailored to your simulation goals.
- Efficiency: Avoid tedious manual editing of index files and seamlessly integrate with GROMACS workflows.
- Flexibility: Use SAMSON’s powerful selection tools to define complex groups effortlessly.
Learn More
Ready to enhance your workflows with custom index groups? Check out the official documentation for more details: Adding custom index groups.
Note: SAMSON and all SAMSON Extensions are free for non-commercial use. Download SAMSON at https://www.samson-connect.net.
